Two methods methods are generally used to detect species-specific viruses in rodent cells:
- Inoculation of animals with the test item, followed by the detection of antibody production serum;
- Detection of the viral genome bu using nucleic acid amplification technology (qPCR).
Mouse, Rat & Hamster Antibody Production (MAP, RAP and HAP) tests
Species-specific viruses present in rodent cell lines can be detected using mouse, rat and hamster antibody production tests. These tests can help select a clone for the production of a recombinant protein or monoclonal antibody.
Different viruses can be screened for, depending on the rodent species used:
|Ectromelia Virus||Lymphocytic Choriomeningitis Virus (LCM)|
|Hantaan Virus||Pneumonia Virus of Mice (PVM)|
|K Virus||Reovirus Type 3 (Reo3)|
|Lactic Dehydrogenase Virus (LDM)||Sendai Virus|
|Lymphocytic Choriomeningitis Virus (LCM)||Simian Virus 5 (SV5)|
|Minute Virus of Mice|
|Mouse Adenovirus (MAV)||RAP|
|Mouse Cytomegalovirus (MCMV)||Hantaan Virus Kilham|
|Mouse Encephalomyelitis Virus (Theilers, GDVII)||Rat Virus (KRV)|
|Mouse Hepatitis Virus (MHV)||Mouse Encephalomyelitis Virus (Theilers, GDVII°)|
|Mouse Rotavirus (EDIM)||Pneumonia Virus of Mice (PVM)|
|Mouse Thymic||Rat Coronavirus (RCV)|
|Pneumonia Virus of Mice (PVM)||Reovirus Type 3 (Reo3)|
|Reovirus Type 3 (Reo 3)||Sialodacryoadenitis Virus (SDA V)|
|Sendai Virus||Toolan Virus (HI)|
Quantitave Polymerase Chain reaction
Texcell has developed an MVM (Minute Virus of Mice)-specific quantitative PCR, because sometimes this virus can be recovered from the final product.
For the following species, Texcell offers qPCR protocols which allowed the detection of more than 20 viruses:
- Human Viruses
- Simian Viruses
- Porcine Viruses
- Bovine Viruses
- Murine Viruses
The qPCR enable rapid detection of specific viruses in test products.