Texcell’s Cell Culture team has nearly 25 years of experience using cell-based systems for protein expression.
A key factor in controlling production costs of biopharmaceutical proteins is the rapid development of recombinant cell lines that produce high levels of expressed protein products.
To help facilitate achieving this goal, our team offers the following important core services to expedite the development process at the R&D level:
- Transfection of recombinant plasmid DNA into mammalian cell lines via lipofectamine or PEI, or transduction of a lentiviral vector
- Stable cell selection using appropriate selective agents (G418, hygromycin, puromycin, etc.)
- Single-cell plating in 96-well plate or cloning by limiting dilution
- Cell clone validation by ELISA or qPCR
- Cryopreservation of the high-level expresser cell line clones for future use
- Preparation of a Research Cell Bank (RCB) from selected clonal cell lines
Using the above approaches, an effective selection strategy can be designed to identify monoclonal cell lines that should stably express high levels of recombinant proteins in culture; these cells will then be ideally suited for transfer to our GMP Cell Banking prior to large-scale production of proteins in standard fed-batch fermentation processes.